Fluid flow facilitates inward rectifier K⁺ current by convectively restoring [K⁺] at the cell membrane surface.

ABSTRACT: The inward rectifier Kir2.1 current (IKir2.1) was reported to be facilitated by fluid flow. However, the mechanism underlying this facilitation remains uncertain. We hypothesized that during K⁺ influx or efflux, [K⁺] adjacent to the outer mouth of the Kir2.1 channel might decrease or increase, respectively, compared with the average [K⁺] of the bulk extracellular solution, and that fluid flow could restore the original [K⁺] and result in the apparent facilitation of IKir2.1. We recorded the IKir2.1 in RBL-2H3 cells and HEK293T cells that were ectopically over-expressed with Kir2.1 channels by using the whole-cell patch-clamp technique. Fluid-flow application immediately increased the IKir2.1, which was not prevented by either the pretreatment wit

SUBMITTER: Kim JG 

PROVIDER: S-EPMC5177964 | biostudies-literature | 2016 Dec

REPOSITORIES: biostudies-literature