The variability and reproducibility of whole genome sequencing technology for detecting resistance to anti-tuberculous drugs.

ABSTRACT:

Background

The emergence of resistance to anti-tuberculosis drugs is a serious and growing threat to public health. Next-generation sequencing is rapidly gaining traction as a diagnostic tool for investigating drug resistance in Mycobacterium tuberculosis to aid treatment decisions. However, there are few little data regarding the precision of such sequencing for assigning resistance profiles.

Methods

We investigated two sequencing platforms (Illumina MiSeq, Ion Torrent PGM™) and two rapid analytic pipelines (TBProfiler, Mykrobe predictor) using a well characterised reference strain (H37Rv) and clinical isolates from patients with tuberculosis resistant to up to 13 drugs. Results were compared to phenotypic drug susceptibility testing. To assess analytical robustness individ

SUBMITTER: Phelan J 

PROVIDER: S-EPMC5178084 | biostudies-literature | 2016 Dec

REPOSITORIES: biostudies-literature