Macroautophagy and Selective Mitophagy Ameliorate Chondrogenic Differentiation Potential in Adipose Stem Cells of Equine Metabolic Syndrome: New Findings in the Field of Progenitor Cells Differentiation.

ABSTRACT: Equine metabolic syndrome (EMS) is mainly characterized by insulin resistance, obesity, and local or systemic inflammation. That unfriendly environment of adipose tissue has huge impact on stem cells population (ASC) residing within. In the present study, using molecular biology techniques and multiple imaging techniques (SEM, FIB-SEM, and confocal microscopy), we evaluated the impact of EMS on ASC viability and chondrogenic differentiation. Moreover, we visualized the mitochondrial network and dynamics in ASC_CTRL and ASC_EMS during control and chondrogenic conditions. In control conditions, ASC_EMS were characterized by increased mitochondrial fission in comparison to ASC_CTRL. We found that extensive remodeling of mitochondrial network including f

SUBMITTER: Marycz K 

PROVIDER: S-EPMC5178365 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature