Antiproliferative and Pro-Apoptotic Effects of MiR-4286 Inhibition in Melanoma Cells.

ABSTRACT:

Introduction

MicroRNAs are essential regulators of gene expression at the post-transcriptional level. Their expression is altered in cancer tissues, and evaluation of these alterations is considered a promising tool used to diagnose and identify prognostic markers.

Materials and methods

The microRNA expression profiles of formalin-fixed, paraffin-embedded melanoma and melanocytic nevi samples were estimated with a microarray and subsequently validated by real-time PCR. Melanoma cells were transfected with miR-4286 inhibitor to evaluate the influence of this microRNA on the viability, proliferation, apoptosis, migration, and invasion of melanoma cells.

Results

The microarray revealed that the expression of 1,171 microRNAs was altered in melanoma samples compared to mel

SUBMITTER: Komina A 

PROVIDER: S-EPMC5179095 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature