Structural Insights on PHA Binding Protein PhaP from Aeromonas hydrophila.

ABSTRACT: Phasins or PhaPs are a group of amphiphilic proteins that are found attached to the surface of microbial polyhydroxyalkanoate (PHA) granules. They have both structural and regulatory functions and can affect intracellular PHA accumulation and mediate protein folding. The molecular basis for the diverse functions of the PhaPs has not been fully understood due to the lack of the structural knowledge. Here we report the structural and biochemical studies of the PhaP cloned from Aeromonas hydrophila (PhaP_Ah), which is utilized in protein and tissue engineering. The crystal structure of PhaP_Ah was revealed to be a tetramer with 8 α-helices adopting a coiled-coil structure. Each monomer has a hydrophobic and a hydrophilic surface, rendering the surfactant properties of the

SUBMITTER: Zhao H 

PROVIDER: S-EPMC5180188 | biostudies-literature | 2016 Dec

REPOSITORIES: biostudies-literature