Development of a high-density genetic linkage map and identification of flowering time QTLs in adzuki bean (Vigna angularis).

ABSTRACT: A high-density linkage map is crucial for the identification of quantitative trait loci (QTLs), positional cloning, and physical map assembly. Here, we report the development of a high-density linkage map based on specific length amplified fragment sequencing (SLAF-seq) for adzuki bean and the identification of flowering time-related QTLs. Through SLAF library construction and Illumina sequencing of a recombinant inbred line (RIL) population, a total of 4425 SLAF markers were developed and assigned to 11 linkage groups (LGs). After binning the SLAF markers that represented the same genotype, the final linkage map of 1628.15 cM contained 2032 markers, with an average marker density of 0.80 cM. Comparative analysis showed high collinearity with two adzuki bean physical maps and a high degree

SUBMITTER: Liu C 

PROVIDER: S-EPMC5180193 | biostudies-literature | 2016 Dec

REPOSITORIES: biostudies-literature