Identification of inhibitors for single-stranded DNA-binding proteins in eubacteria.

ABSTRACT:

Objectives

The increasing threat of drug-resistant bacteria establishes a continuing need for the development of new strategies to fight infection. We examine the inhibition of the essential single-stranded DNA-binding proteins (SSBs) SSBA and SSBB as a potential antimicrobial therapy due to their importance in DNA replication, activating the SOS response and promoting competence-based mechanisms of resistance by incorporating new DNA.

Methods

Purified recombinant SSBs from Gram-positive (Staphylococcus aureus and Bacillus anthracis) and Gram-negative (Escherichia coli and Francisella tularensis) bacteria were assessed in a high-throughput screen for inhibition of duplex DNA unwinding by small molecule inhibitors. Secondary electrophoretic mobility shift assays further valid

SUBMITTER: Glanzer JG 

PROVIDER: S-EPMC5181397 | biostudies-literature | 2016 Dec

REPOSITORIES: biostudies-literature