Development of tools to automate quantitative analysis of radiation damage in SAXS experiments.

ABSTRACT: Biological small-angle X-ray scattering (SAXS) is an increasingly popular technique used to obtain nanoscale structural information on macromolecules in solution. However, radiation damage to the samples limits the amount of useful data that can be collected from a single sample. In contrast to the extensive analytical resources available for macromolecular crystallography (MX), there are relatively few tools to quantitate radiation damage for SAXS, some of which require a significant level of manual characterization, with the potential of leading to conflicting results from different studies. Here, computational tools have been developed to automate and standardize radiation damage analysis for SAXS data. RADDOSE-3D, a dose calculation software utility originally written for MX experiment

SUBMITTER: Brooks-Bartlett JC 

PROVIDER: S-EPMC5182020 | biostudies-literature | 2017 Jan

REPOSITORIES: biostudies-literature