B-cell populations discriminate between pediatric- and adult-onset multiple sclerosis.

ABSTRACT:

Objective

To comparatively assess the B-cell composition in blood and CSF of patients with pediatric-onset multiple sclerosis (pedMS) and adult-onset multiple sclerosis (adMS).

Methods

In this cross-sectional study, we obtained blood and CSF samples from 25 patients with pedMS (8-18 years) and 40 patients with adMS (23-65 years) and blood specimens from 66 controls (1-55 years). By using multicolor flow cytometry, we identified naive, transitional, isotype class-switched memory, nonswitched memory, and double-negative memory B-cell subsets as well as plasmablasts (PB) and terminally differentiated plasma cells (PC). Flow cytometric data were compared to concentrations of B-cell-specific cytokines in serum and CSF as determined by ELISA.

Results

Frequencies of circulat

SUBMITTER: Schwarz A 

PROVIDER: S-EPMC5182056 | biostudies-literature | 2017 Jan

REPOSITORIES: biostudies-literature