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Characterizing polyubiquitinated forms of the neurodegenerative ubiquitin mutant UBB⁺¹.

ABSTRACT: The ubiquitin mutant UBB⁺¹ has been identified as a hallmark of neurodegenerative diseases. In this study, we characterize polyubiquitinated forms of UBB⁺¹ in vitro and from patient samples. The ability of UBB⁺¹ to be readily ubiquitinated by several E2 enzymes provided a mechanism for the controlled synthesis and purification of defined conjugates. This allowed us to utilize polyUb-UBB⁺¹ conjugates for biochemical assays, as well as solution NMR. Coupled with our immunoassay for detection of ubiquitinated forms of UBB⁺¹ in patient blood samples, we gain a clearer picture of the molecular mechanisms underlying neurodegenerative diseases.

SUBMITTER: Chojnacki M

PROVIDER: S-EPMC5182158 | biostudies-literature | 2016 Dec

REPOSITORIES: biostudies-literature

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Characterizing polyubiquitinated forms of the neurodegenerative ubiquitin mutant UBB⁺¹.

OmicsDI is part of the ELIXIR infrastructure

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Dataset Information

Characterizing polyubiquitinated forms of the neurodegenerative ubiquitin mutant UBB+1.

OmicsDI is part of the ELIXIR infrastructure

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Characterizing polyubiquitinated forms of the neurodegenerative ubiquitin mutant UBB⁺¹.