Long-Term Alteration of Reactive Oxygen Species Led to Multidrug Resistance in MCF-7 Cells.

ABSTRACT: Reactive oxygen species (ROS) play an important role in multidrug resistance (MDR). This study aimed to investigate the effects of long-term ROS alteration on MDR in MCF-7 cells and to explore its underlying mechanism. Our study showed both long-term treatments of H₂O₂ and glutathione (GSH) led to MDR with suppressed iROS levels in MCF-7 cells. Moreover, the MDR cells induced by 0.1 μM H₂O₂ treatment for 20 weeks (MCF-7/ROS cells) had a higher viability and proliferative ability than the control MCF-7 cells. MCF-7/ROS cells also showed higher activity or content of intracellular antioxidants like glutathione peroxidase (GPx), GSH, superoxide dismutase (SOD), and catalase (CAT). Importantly, MCF-7/ROS cells were characterized by overexpress

SUBMITTER: Cen J 

PROVIDER: S-EPMC5183793 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature