Discrimination of Paederus fuscipes and Paederus littoralis by mtDNA-COI PCR-RFLP.

ABSTRACT:

Background

Linear dermatitis is endemic in Iran where most cases occur in the Caspian Sea coast and Fars province. The disease is caused by beetles of the genus Paederus which are active from early spring to beginning of autumn although its incidence rises from May to August. The classic taxonomy of Paederus spp. is based on the male genitalia that is very complex and needs expertise. In this study, we report a DNA-based method to discriminate Paederus fuscipes and Paederus littoralis (=syn: P. lenkoranus, P. ilsae).

Methods

Type specimens were collected from north and south of Iran. Molecular typing of the species was performed using restriction fragment length polymorphism (RFLP) analysis of polymerase chain reaction (PCR)-amplifie

SUBMITTER: Bazrafkan S 

PROVIDER: S-EPMC5186735 | biostudies-literature | 2016 Dec

REPOSITORIES: biostudies-literature