Unknown

Dataset Information

0

Optic Atrophy 1 Is Epistatic to the Core MICOS Component MIC60 in Mitochondrial Cristae Shape Control.

ABSTRACT: The mitochondrial contact site and cristae organizing system (MICOS) and Optic atrophy 1 (OPA1) control cristae shape, thus affecting mitochondrial function and apoptosis. Whether and how they physically and functionally interact is unclear. Here, we provide evidence that OPA1 is epistatic to MICOS in the regulation of cristae shape. Proteomic analysis identifies multiple MICOS components in native OPA1-containing high molecular weight complexes disrupted during cristae remodeling. MIC60, a core MICOS protein, physically interacts with OPA1, and together, they control cristae junction number and stability, OPA1 being epistatic to MIC60. OPA1 defines cristae width and junction diameter independently of MIC60. Our combination of proteomics, biochemistry, genetics, and electron tomography provides a unifying model for mammalian cristae biogenesis by OPA1 and MICOS.

SUBMITTER: Glytsou C 

PROVIDER: S-EPMC5186903 | biostudies-literature | 2016 Dec

REPOSITORIES: biostudies-literature

Json Xml
altmetric image

Publications

The mitochondrial contact site and cristae organizing system (MICOS) and Optic atrophy 1 (OPA1) control cristae shape, thus affecting mitochondrial function and apoptosis. Whether and how they physically and functionally interact is unclear. Here, we provide evidence that OPA1 is epistatic to MICOS in the regulation of cristae shape. Proteomic analysis identifies multiple MICOS components in native OPA1-containing high molecular weight complexes disrupted during cristae remodeling. MIC60, a core  ...[more]

Similar Datasets

Unknown The MICOS component Mic60 displays a conserved membrane-bending activity that is necessary for normal cristae morphology.
| S-EPMC5379949 | biostudies-literature
Unknown The cristae modulator Optic atrophy 1 requires mitochondrial ATP synthase oligomers to safeguard mitochondrial function.
| S-EPMC6109181 | biostudies-literature
Unknown Mic60/Mitofilin determines MICOS assembly essential for mitochondrial dynamics and mtDNA nucleoid organization.
| S-EPMC5072434 | biostudies-literature
Unknown Sam50-Mic19-Mic60 axis determines mitochondrial cristae architecture by mediating mitochondrial outer and inner membrane contact.
| S-EPMC7206006 | biostudies-literature
Unknown MICOS assembly controls mitochondrial inner membrane remodeling and crista junction redistribution to mediate cristae formation.
| S-EPMC7361284 | biostudies-literature
Unknown The MICOS Complex Subunit Mic60 is Hijacked by Intracellular Bacteria to Manipulate Mitochondrial Dynamics and Promote Bacterial Pathogenicity.
| S-EPMC11633497 | biostudies-literature
Unknown MICOS assembly controls mitochondrial inner membrane remodeling and crista junction redistribution to mediate cristae formation
| S-SCDT-EMBOJ-2019-104105 | biostudies-other
Unknown Mitochondrial contact site and cristae organizing system (MICOS) machinery supports heme biosynthesis by enabling optimal performance of ferrochelatase.
| S-EPMC8441213 | biostudies-literature
Unknown A DRP-like pseudoenzyme coordinates with MICOS to promote cristae architecture.
| S-EPMC10592917 | biostudies-literature
Unknown Mitochondrial cristae shape determines respiratory chain supercomplexes assembly and respiratory efficiency.
| S-EPMC3790458 | biostudies-literature