Complementarity of PALM and SOFI for super-resolution live-cell imaging of focal adhesions.

ABSTRACT: Live-cell imaging of focal adhesions requires a sufficiently high temporal resolution, which remains a challenge for super-resolution microscopy. Here we address this important issue by combining photoactivated localization microscopy (PALM) with super-resolution optical fluctuation imaging (SOFI). Using simulations and fixed-cell focal adhesion images, we investigate the complementarity between PALM and SOFI in terms of spatial and temporal resolution. This PALM-SOFI framework is used to image focal adhesions in living cells, while obtaining a temporal resolution below 10 s. We visualize the dynamics of focal adhesions, and reveal local mean velocities around 190 nm min^-1. The complementarity of PALM and SOFI is assessed in detail with a methodology that integrates a resolution

SUBMITTER: Deschout H 

PROVIDER: S-EPMC5187410 | biostudies-literature | 2016 Dec

REPOSITORIES: biostudies-literature