Impact of the gut microbiota on enhancer accessibility in gut intraepithelial lymphocytes.

ABSTRACT: The gut microbiota impacts many aspects of host biology including immune function. One hypothesis is that microbial communities induce epigenetic changes with accompanying alterations in chromatin accessibility, providing a mechanism that allows a community to have sustained host effects even in the face of its structural or functional variation. We used Assay for Transposase-Accessible Chromatin with high-throughput sequencing (ATAC-seq) to define chromatin accessibility in predicted enhancer regions of intestinal αβ⁺ and γδ⁺ intraepithelial lymphocytes purified from germ-free mice, their conventionally raised (CONV-R) counterparts, and mice reared germ free and then colonized with CONV-R gut microbiota at the end of the suckling-weaning transition. Characterizing ge

SUBMITTER: Semenkovich NP 

PROVIDER: S-EPMC5187723 | biostudies-literature | 2016 Dec

REPOSITORIES: biostudies-literature