Distinct roles of transcription factors TFIIIB and TFIIIC in RNA polymerase III transcription reinitiation.

ABSTRACT: Eukaryotic RNA polymerase (Pol) III is recruited to target promoters by a stable preinitiation complex containing transcription factors TFIIIC and TFIIIB. After the first transcription cycle, reinitiation proceeds through facilitated recycling, a process by which the terminating Pol III rapidly reloads onto the same transcription unit. Here, we show that Pol III is repeatedly recaptured in vitro by the first transcribed gene, even in the presence of a juxtaposed competitor promoter complex, thus suggesting that facilitated recycling is not merely due to a stochastic reassociation process favored by the small size of class III genes. The transcription factor requirements for facilitated reinitiation were investigated by taking advantage of Pol III templates that support both TFIIIC-dependen

SUBMITTER: Ferrari R 

PROVIDER: S-EPMC518776 | biostudies-literature | 2004 Sep

REPOSITORIES: biostudies-literature