Down-Regulation of Ca²⁺-Activated K⁺ Channel K_Ca1.1 in Human Breast Cancer MDA-MB-453 Cells Treated with Vitamin D Receptor Agonists.

ABSTRACT: Vitamin D (VD) reduces the risk of breast cancer and improves disease prognoses. Potential VD analogs are being developed as therapeutic agents for breast cancer treatments. The large-conductance Ca²⁺-activated K⁺ channel K_Ca1.1 regulates intracellular Ca²⁺ signaling pathways and is associated with high grade tumors and poor prognoses. In the present study, we examined the effects of treatments with VD receptor (VDR) agonists on the expression and activity of K_Ca1.1 in human breast cancer MDA-MB-453 cells using real-time PCR, Western blotting, flow cytometry, and voltage-sensitive dye imaging. Treatments with VDR agonists for 72 h markedly decreased the expression levels of K_Ca1.1 transcripts and proteins in MDA-MB-453 cells, resulting

SUBMITTER: Khatun A 

PROVIDER: S-EPMC5187883 | biostudies-literature | 2016 Dec

REPOSITORIES: biostudies-literature