Dynamic alterations of membrane tethers stabilize leukocyte rolling on P-selectin.

ABSTRACT: Leukocytes rolling on selectins extrude thin membrane tethers that might stabilize rolling velocities despite marked alterations in wall shear stress. To test this hypothesis, we used differential interference contrast videomicroscopy to visualize formation and breakage of membrane tethers as neutrophils rolled on P-selectin under flow. Neutrophils rapidly increased tether number as wall shear stress rose and decreased tether number as wall shear stress declined. Membrane tethers invariably accompanied slower, more uniform rolling steps that translated into lower mean rolling velocities and variances in velocity. Unexpectedly, neutrophils, but not fixed cells or microspheres bearing selectin ligands, rolled progressively more slowly and uniformly over time. Scanning electron microscopy rev

SUBMITTER: Ramachandran V 

PROVIDER: S-EPMC518789 | biostudies-literature | 2004 Sep

REPOSITORIES: biostudies-literature