Mesenchymal Stem Cell-Derived Exosomes Promote Fracture Healing in a Mouse Model.

ABSTRACT: : Paracrine signaling by bone-marrow-derived mesenchymal stem cells (MSCs) plays a major role in tissue repair. Although the production of regulatory cytokines by MSC transplantation is a critical modulator of tissue regeneration, we focused on exosomes, which are extracellular vesicles that contain proteins and nucleic acids, as a novel additional modulator of cell-to-cell communication and tissue regeneration. To address this, we used radiologic imaging, histological examination, and immunohistochemical analysis to evaluate the role of exosomes isolated from MSC-conditioned medium (CM) in the healing process in a femur fracture model of CD9^-/- mice, a strain that is known to produce reduced levels of exosomes. We found that the bone union rate in CD9^-/- mice was sig

SUBMITTER: Furuta T 

PROVIDER: S-EPMC5189643 | biostudies-literature | 2016 Dec

REPOSITORIES: biostudies-literature