ARNA-longSAGE: a new approach to generate SAGE libraries from microdissected cells.

ABSTRACT: Large-scale gene expression analyses of microdissected primary tissue are still difficult because generally only a limited amount of mRNA can be obtained from microdissected cells. The introduction of the T7-based RNA amplification technique was an important step to reduce the amount of RNA needed for such analyses. This amplification technique produces amplified antisense RNA (aRNA), which so far has precluded its direct use for serial analysis of gene expression (SAGE) library production. We describe a method, termed 'aRNA-longSAGE', which is the first to allow the direct use of aRNA for standard longSAGE library production. The aRNA-longSAGE protocol was validated by comparing two aRNA-longSAGE libraries with two Micro-longSAGE libraries that were generated from the same RNA preparation

SUBMITTER: Heidenblut AM 

PROVIDER: S-EPMC519129 | biostudies-literature | 2004 Sep

REPOSITORIES: biostudies-literature