Monitoring acute metabolic changes in the liver and kidneys induced by fructose and glucose using hyperpolarized [2-¹³ C]dihydroxyacetone.

ABSTRACT:

Purpose

To investigate acute changes in glucose metabolism in liver and kidneys in vivo after a bolus injection of either fructose or glucose, using hyperpolarized [2-¹³ C]dihydroxyacetone.

Methods

Spatially registered, dynamic, multislice MR spectroscopy was acquired for the metabolic products of [2-¹³ C]dihydroxyacetone in liver and kidneys. Metabolism was probed in 13 fasted rats at three time points: 0, 70, and 140 min. At 60 min, rats were injected intravenously with fructose (n = 5) or glucose (n = 4) at 0.8 g/kg to initiate acute response. Controls (n = 4) did not receive a carbohydrate challenge.

Results

Ten minutes after fructose infusion, levels of [2-¹³ C]phosphoenolpyruvate and [2-¹³ C]glycerol-3-phosphate halved

SUBMITTER: Marco-Rius I 

PROVIDER: S-EPMC5191913 | biostudies-literature | 2017 Jan

REPOSITORIES: biostudies-literature