Gene expression profiling of U2AF2 dependent RNA-protein interactions during CD4 + T cell activation.

ABSTRACT: CD4 T cell activation is a central component of the mammalian adaptive immune response and is underscored by a dramatic change in the gene expression profile in these cells. The changes in gene expression that occur during T cell activation are regulated in multiple ways including post-transcriptionally by complexes of RNA-binding proteins. Recently, our study explored the role of the RNA-binding protein U2AF2 and its interacting proteins in mediating posttranscriptional changes in constitutive and alternative splicing during T cell activation. First, we used RNA-seq to identify the global changes in gene expression and splicing that occur with T cell activation. Next, we used RIP-seq to identify the specific genes bound to U2AF2 during T cell activation. After identification of the protei

SUBMITTER: Whisenant TC 

PROVIDER: S-EPMC5192059 | biostudies-literature | 2017 Mar

REPOSITORIES: biostudies-literature