Optimization of Cyanine Dye Stability and Analysis of FRET Interaction on DNA Microarrays.

ABSTRACT: The application of DNA microarrays for high throughput analysis of genetic regulation is often limited by the fluorophores used as markers. The implementation of multi-scan techniques is limited by the fluorophores' susceptibility to photobleaching when exposed to the scanner laser light. This paper presents combined mechanical and chemical strategies which enhance the photostability of cyanine 3 and cyanine 5 as part of solid state DNA microarrays. These strategies are based on scanning the microarrays while the hybridized DNA is still in an aqueous solution with the presence of a reductive/oxidative system (ROXS). Furthermore, the experimental setup allows for the analysis and eventual normalization of Förster-resonance-energy-transfer (FRET) interaction of cyanine-3/cyanine-5 dye combin

SUBMITTER: von der Haar M 

PROVIDER: S-EPMC5192427 | biostudies-literature | 2016 Nov

REPOSITORIES: biostudies-literature