Dynamic properties of independent chromatin domains measured by correlation spectroscopy in living cells.

ABSTRACT:

Background

Genome organization into subchromosomal topologically associating domains (TADs) is linked to cell-type-specific gene expression programs. However, dynamic properties of such domains remain elusive, and it is unclear how domain plasticity modulates genomic accessibility for soluble factors.

Results

Here, we combine and compare a high-resolution topology analysis of interacting chromatin loci with fluorescence correlation spectroscopy measurements of domain dynamics in single living cells. We identify topologically and dynamically independent chromatin domains of ~1 Mb in size that are best described by a loop-cluster polymer model. Hydrodynamic relaxation times and gyration radii of domains are larger for open (161 ± 15 ms, 297 ± 9 nm) than for dense chromatin (88

SUBMITTER: Wachsmuth M 

PROVIDER: S-EPMC5192577 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature