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Atomic resolution structure determination by the cryo-EM method MicroED.

ABSTRACT: The electron cryo-microscopy (cryoEM) method MicroED has been rapidly developing. In this review we highlight some of the key steps in MicroED from crystal analysis to structure determination. We compare and contrast MicroED and the latest X-ray based diffraction method the X-ray free-electron laser (XFEL). Strengths and shortcomings of both MicroED and XFEL are discussed. Finally, all current MicroED structures are tabulated with a view to the future.

SUBMITTER: Liu S 

PROVIDER: S-EPMC5192978 | biostudies-literature | 2017 Jan

REPOSITORIES: biostudies-literature

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Atomic resolution structure determination by the cryo-EM method MicroED.

Liu Shian S   Hattne Johan J   Reyes Francis E FE   Sanchez-Martinez Silvia S   Jason de la Cruz M M   Shi Dan D   Gonen Tamir T  

Protein science : a publication of the Protein Society 20160819 1

The electron cryo-microscopy (cryoEM) method MicroED has been rapidly developing. In this review we highlight some of the key steps in MicroED from crystal analysis to structure determination. We compare and contrast MicroED and the latest X-ray based diffraction method the X-ray free-electron laser (XFEL). Strengths and shortcomings of both MicroED and XFEL are discussed. Finally, all current MicroED structures are tabulated with a view to the future. ...[more]

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