High-Content Imaging Platform for Profiling Intracellular Signaling Network Activity in Living Cells.

ABSTRACT: Essential characteristics of cellular signaling networks include a complex interconnected architecture and temporal dynamics of protein activity. The latter can be monitored by Förster resonance energy transfer (FRET) biosensors at a single-live-cell level with high temporal resolution. However, these experiments are typically limited to the use of a couple of FRET biosensors. Here, we describe a FRET-based multi-parameter imaging platform (FMIP) that allows simultaneous high-throughput monitoring of multiple signaling pathways. We apply FMIP to monitor the crosstalk between epidermal growth factor receptor (EGFR) and insulin-like growth factor-1 receptor signaling, signaling perturbations caused by pathophysiologically relevant EGFR mutations, and the effects of a clinically important MEK

SUBMITTER: Kuchenov D 

PROVIDER: S-EPMC5193178 | biostudies-literature | 2016 Dec

REPOSITORIES: biostudies-literature