A quantitative LC-MS/MS method for simultaneous determination of cocaine and its metabolites in whole blood.

ABSTRACT: As new metabolic pathways of cocaine were recently identified, a high performance liquid chromatography tandem mass spectrometry (LC-MS/MS) method was developed to simultaneously determine cocaine and nine cocaine-related metabolites in whole blood samples. One-step solid phase extraction was used to extract all of the ten compounds and corresponding internal standards from blood samples. All compounds and internal standards extracted were separated on an Atlantis T3 (100Å, 3μm, 2.1mm×150mm I.D) column and detected in positive ion and high sensitivity mode with multiple reaction monitoring. This method was validated for its sensitivity, linearity, specificity, accuracy, precision, recovery, and stability. All of the ten compounds were quantifiable ranging from the lower limit of quantifica

SUBMITTER: Chen X 

PROVIDER: S-EPMC5196007 | biostudies-literature | 2017 Feb

REPOSITORIES: biostudies-literature