Droplet Microarray Based on Superhydrophobic-Superhydrophilic Patterns for Single Cell Analysis.

ABSTRACT: Single-cell analysis provides fundamental information on individual cell response to different environmental cues and is a growing interest in cancer and stem cell research. However, current existing methods are still facing challenges in performing such analysis in a high-throughput manner whilst being cost-effective. Here we established the Droplet Microarray (DMA) as a miniaturized screening platform for high-throughput single-cell analysis. Using the method of limited dilution and varying cell density and seeding time, we optimized the distribution of single cells on the DMA. We established culturing conditions for single cells in individual droplets on DMA obtaining the survival of nearly 100% of single cells and doubling time of single cells comparable with that of cells cultured in

SUBMITTER: Jogia GE 

PROVIDER: S-EPMC5197947 | biostudies-literature | 2016 Dec

REPOSITORIES: biostudies-literature