Bioprinting 3D microfibrous scaffolds for engineering endothelialized myocardium and heart-on-a-chip.

ABSTRACT: Engineering cardiac tissues and organ models remains a great challenge due to the hierarchical structure of the native myocardium. The need of integrating blood vessels brings additional complexity, limiting the available approaches that are suitable to produce integrated cardiovascular organoids. In this work we propose a novel hybrid strategy based on 3D bioprinting, to fabricate endothelialized myocardium. Enabled by the use of our composite bioink, endothelial cells directly bioprinted within microfibrous hydrogel scaffolds gradually migrated towards the peripheries of the microfibers to form a layer of confluent endothelium. Together with controlled anisotropy, this 3D endothelial bed was then seeded with cardiomyocytes to generate aligned myocardium capable of spontaneous and synchro

SUBMITTER: Zhang YS 

PROVIDER: S-EPMC5198581 | biostudies-literature | 2016 Dec

REPOSITORIES: biostudies-literature