Increased fibroblast chymase production mediates procollagen autophagic digestion in volume overload.

ABSTRACT:

Background

Previous work has identified mast cells as the major source of chymase largely associated with a profibrotic phenotype. We recently reported increased fibroblast autophagic procollagen degradation in a rat model of pure volume overload (VO). Here we demonstrate a connection between increased fibroblast chymase production and autophagic digestion of procollagen in the pure VO of aortocaval fistula (ACF) in the rat.

Methods and results

Isolated LV fibroblasts taken from 4 and 12week ACF Sprague-Dawley rats have significant increases in chymase mRNA and chymase activity. Increased intracellular chymase protein is documented by immunocytochemistry in the ACF fibroblasts compared to cells obtained from age-matched sham rats. To implicate VO as a stimulus for chymase pr

SUBMITTER: Fu L 

PROVIDER: S-EPMC5198899 | biostudies-literature | 2016 Mar

REPOSITORIES: biostudies-literature