Diverse mechanisms of metaeffector activity in an intracellular bacterial pathogen, Legionella pneumophila.

ABSTRACT: Pathogens deliver complex arsenals of translocated effector proteins to host cells during infection, but the extent to which these proteins are regulated once inside the eukaryotic cell remains poorly defined. Among all bacterial pathogens, Legionella pneumophila maintains the largest known set of translocated substrates, delivering over 300 proteins to the host cell via its Type IVB, Icm/Dot translocation system. Backed by a few notable examples of effector-effector regulation in L. pneumophila, we sought to define the extent of this phenomenon through a systematic analysis of effector-effector functional interaction. We used Saccharomyces cerevisiae, an established proxy for the eukaryotic host, to query > 108,000 pairwise genetic interactions between two compatible expression libraries

SUBMITTER: Urbanus ML 

PROVIDER: S-EPMC5199130 | biostudies-literature | 2016 Dec

REPOSITORIES: biostudies-literature