Lysozyme association with circulating RNA, extracellular vesicles, and chronic stress.

ABSTRACT:

Background

Stress has demonstrated effects on inflammation though underlying cell-cell communication mechanisms remain unclear. We hypothesize that circulating RNAs and extracellular vesicles (EVs) in patients with chronic stress contain signals with functional roles in cell repair.

Methods

Blood transcriptome from patients with Irritable Bowel Syndrome versus controls were compared to identify signaling pathways and effectors. Plasma EVs were isolated (size-exclusion chromatography) and characterized for effectors' presence (immunogold labelling-electron microscopy). Based on transcriptome pathways and EV-labelling, lysozyme's effects on cell migration were tested in human colon epithelial CRL-1790 cells and compared to the effects of CXCL12, a migration inducer (wound assa

SUBMITTER: Abey SK 

PROVIDER: S-EPMC5200883 | biostudies-literature | 2017 Jun

REPOSITORIES: biostudies-literature