An Improved Methodology to Overcome Key Issues in Human Fecal Metagenomic DNA Extraction.

ABSTRACT: Microbes are ubiquitously distributed in nature, and recent culture-independent studies have highlighted the significance of gut microbiota in human health and disease. Fecal DNA is the primary source for the majority of human gut microbiome studies. However, further improvement is needed to obtain fecal metagenomic DNA with sufficient amount and good quality but low host genomic DNA contamination. In the current study, we demonstrate a quick, robust, unbiased, and cost-effective method for the isolation of high molecular weight (>23kb) metagenomic DNA (260/280 ratio >1.8) with a good yield (55.8±3.8ng/mg of feces). We also confirm that there is very low human genomic DNA contamination (eubacterial: human genomic DNA marker genes=2^27.9:1) in the human feces. The newly-developed

SUBMITTER: Kumar J 

PROVIDER: S-EPMC5200916 | biostudies-literature | 2016 Dec

REPOSITORIES: biostudies-literature