Specification of osteoblast cell fate by canonical Wnt signaling requires Bmp2.

ABSTRACT: Enhanced BMP or canonical Wnt (cWnt) signaling are therapeutic strategies employed to enhance bone formation and fracture repair, but the mechanisms each pathway utilizes to specify cell fate of bone-forming osteoblasts remain poorly understood. Among all BMPs expressed in bone, we find that singular deficiency of Bmp2 blocks the ability of cWnt signaling to specify osteoblasts from limb bud or bone marrow progenitors. When exposed to cWnts, Bmp2-deficient cells fail to progress through the Runx2/Osx1 checkpoint and thus do not upregulate multiple genes controlling mineral metabolism in osteoblasts. Cells lacking Bmp2 after induction of Osx1 differentiate normally in response to cWnts, suggesting that pre-Osx1⁺ osteoprogenitors are an essential source and a target of BMP2. Our a

SUBMITTER: Salazar VS 

PROVIDER: S-EPMC5201040 | biostudies-literature | 2016 Dec

REPOSITORIES: biostudies-literature