Claudin-2-mediated cation and water transport share a common pore.

ABSTRACT:

Aim

Claudin-2 is a tight junction protein typically located in 'leaky' epithelia exhibiting large paracellular permeabilities like small intestine and proximal kidney tubule. Former studies revealed that claudin-2 forms paracellular channels for small cations like sodium and potassium and also paracellular channels for water. This study analyses whether the diffusive transport of sodium and water occurs through a common pore of the claudin-2 channel.

Methods

Wild-type claudin-2 and different claudin-2 mutants were expressed in MDCK I kidney tubule cells using an inducible system. Ion and water permeability and the effect of blocking reagents on both were investigated on different clones of the mutants.

Results

Neutralization of a negatively charged cation interaction

SUBMITTER: Rosenthal R 

PROVIDER: S-EPMC5201457 | biostudies-literature | 2017 Feb

REPOSITORIES: biostudies-literature