Integrating transcriptomic and proteomic data for accurate assembly and annotation of genomes.

ABSTRACT: Complementing genome sequence with deep transcriptome and proteome data could enable more accurate assembly and annotation of newly sequenced genomes. Here, we provide a proof-of-concept of an integrated approach for analysis of the genome and proteome of Anopheles stephensi, which is one of the most important vectors of the malaria parasite. To achieve broad coverage of genes, we carried out transcriptome sequencing and deep proteome profiling of multiple anatomically distinct sites. Based on transcriptomic data alone, we identified and corrected 535 events of incomplete genome assembly involving 1196 scaffolds and 868 protein-coding gene models. This proteogenomic approach enabled us to add 365 genes that were missed during genome annotation and identify 917 gene correction events throug

SUBMITTER: Prasad TS 

PROVIDER: S-EPMC5204337 | biostudies-literature | 2017 Jan

REPOSITORIES: biostudies-literature