The dynamic landscape of fission yeast meiosis alternative-splice isoforms.

ABSTRACT: Alternative splicing increases the diversity of transcriptomes and proteomes in metazoans. The extent to which alternative splicing is active and functional in unicellular organisms is less understood. Here, we exploit a single-molecule long-read sequencing technique and develop an open-source software program called SpliceHunter to characterize the transcriptome in the meiosis of fission yeast. We reveal 14,353 alternative splicing events in 17,669 novel isoforms at different stages of meiosis, including antisense and read-through transcripts. Intron retention is the major type of alternative splicing, followed by alternate "intron in exon." Seven hundred seventy novel transcription units are detected; 53 of the predicted proteins show homology in other species and form theoretical stable

SUBMITTER: Kuang Z 

PROVIDER: S-EPMC5204338 | biostudies-literature | 2017 Jan

REPOSITORIES: biostudies-literature