A systematic comparison reveals substantial differences in chromosomal versus episomal encoding of enhancer activity.

ABSTRACT: Candidate enhancers can be identified on the basis of chromatin modifications, the binding of chromatin modifiers and transcription factors and cofactors, or chromatin accessibility. However, validating such candidates as bona fide enhancers requires functional characterization, typically achieved through reporter assays that test whether a sequence can increase expression of a transcriptional reporter via a minimal promoter. A longstanding concern is that reporter assays are mainly implemented on episomes, which are thought to lack physiological chromatin. However, the magnitude and determinants of differences in cis-regulation for regulatory sequences residing in episomes versus chromosomes remain almost completely unknown. To address this systematically, we developed and applied a novel

SUBMITTER: Inoue F 

PROVIDER: S-EPMC5204343 | biostudies-literature | 2017 Jan

REPOSITORIES: biostudies-literature