Nucleosome fragility is associated with future transcriptional response to developmental cues and stress in C. elegans.

ABSTRACT: Nucleosomes have structural and regulatory functions in all eukaryotic DNA-templated processes. The position of nucleosomes on DNA and the stability of the underlying histone-DNA interactions affect the access of regulatory proteins to DNA. Both stability and position are regulated through DNA sequence, histone post-translational modifications, histone variants, chromatin remodelers, and transcription factors. Here, we explored the functional implications of nucleosome properties on gene expression and development in Caenorhabditis elegans embryos. We performed a time-course of micrococcal nuclease (MNase) digestion and measured the relative sensitivity or resistance of nucleosomes throughout the genome. Fragile nucleosomes were defined by nucleosomal DNA fragments that were recovered pref

SUBMITTER: Jeffers TE 

PROVIDER: S-EPMC5204346 | biostudies-literature | 2017 Jan

REPOSITORIES: biostudies-literature