Large-scale mapping of gene regulatory logic reveals context-dependent repression by transcriptional activators.

ABSTRACT: Transcription factors (TFs) are key mediators that propagate extracellular and intracellular signals through to changes in gene expression profiles. However, the rules by which promoters decode the amount of active TF into target gene expression are not well understood. To determine the mapping between promoter DNA sequence, TF concentration, and gene expression output, we have conducted in budding yeast a large-scale measurement of the activity of thousands of designed promoters at six different levels of TF. We observe that maximum promoter activity is determined by TF concentration and not by the number of binding sites. Surprisingly, the addition of an activator site often reduces expression. A thermodynamic model that incorporates competition between neighboring binding sites for a lo

SUBMITTER: van Dijk D 

PROVIDER: S-EPMC5204347 | biostudies-literature | 2017 Jan

REPOSITORIES: biostudies-literature