L-Plastin promotes podosome longevity and supports macrophage motility.

ABSTRACT: Elucidating the molecular regulation of macrophage migration is essential for understanding the pathophysiology of multiple human diseases, including host responses to infection and autoimmune disorders. Macrophage migration is supported by dynamic rearrangements of the actin cytoskeleton, with formation of actin-based structures such as podosomes and lamellipodia. Here we provide novel insights into the function of the actin-bundling protein l-plastin (LPL) in primary macrophages. We found that podosome stability is disrupted in primary resident peritoneal macrophages from LPL^-/- mice. Live-cell imaging of F-actin using resident peritoneal macrophages from LifeACT-RFP⁺ mice demonstrated that loss of LPL led to decreased longevity of podosomes, without reducing the nu

SUBMITTER: Zhou JY 

PROVIDER: S-EPMC5205537 | biostudies-literature | 2016 Oct

REPOSITORIES: biostudies-literature