Transcriptome profile of one-month-old lambs' granulosa cells after superstimulation.

ABSTRACT:

Objective

Superstimulatory treatment of one-month-old lambs can achieve synchronous development of numerous growing follicles. However, these growing follicles cannot complete maturation and ovulation. Oocyte maturation and competence are acquired during follicular development, in which granulosa cells play an essential role.

Methods

In this study, we applied RNA sequencing to analyze and compare gene expression between prepubertal and adult superstimulated follicle granulosa cells in sheep.

Results

There were more than 300 genes that significantly differed in expression. Among these differently expressed genes, many extracellular matrix genes (EGF containing Fibulin Like Extracellular Matrix Protein 1, pentraxin 3, adrenomedullin, and osteopontin) were significantly

SUBMITTER: Wu Y 

PROVIDER: S-EPMC5205588 | biostudies-literature | 2017 Jan

REPOSITORIES: biostudies-literature