Clarifying intact 3D tissues on a microfluidic chip for high-throughput structural analysis.

ABSTRACT: On-chip imaging of intact three-dimensional tissues within microfluidic devices is fundamentally hindered by intratissue optical scattering, which impedes their use as tissue models for high-throughput screening assays. Here, we engineered a microfluidic system that preserves and converts tissues into optically transparent structures in less than 1 d, which is 20× faster than current passive clearing approaches. Accelerated clearing was achieved because the microfluidic system enhanced the exchange of interstitial fluids by 567-fold, which increased the rate of removal of optically scattering lipid molecules from the cross-linked tissue. Our enhanced clearing process allowed us to fluorescently image and map the segregation and compartmentalization of different cells during the formation o

SUBMITTER: Chen YY 

PROVIDER: S-EPMC5206515 | biostudies-literature | 2016 Dec

REPOSITORIES: biostudies-literature