Transient Kinetics Define a Complete Kinetic Model for Protein Arginine Methyltransferase 1.

ABSTRACT: Protein arginine methyltransferases (PRMTs) are the enzymes responsible for posttranslational methylation of protein arginine residues in eukaryotic cells, particularly within the histone tails. A detailed mechanistic model of PRMT-catalyzed methylation is currently lacking, but it is essential for understanding the functions of PRMTs in various cellular pathways and for efficient design of PRMT inhibitors as potential treatments for a range of human diseases. In this work, we used stopped-flow fluorescence in combination with global kinetic simulation to dissect the transient kinetics of PRMT1, the predominant type I arginine methyltransferase. Several important mechanistic insights were revealed. The cofactor and the peptide substrate bound to PRMT1 in a random manner and then followed a

SUBMITTER: Hu H 

PROVIDER: S-EPMC5207181 | biostudies-literature | 2016 Dec

REPOSITORIES: biostudies-literature