Power Stroke Angular Velocity Profiles of Archaeal A-ATP Synthase Versus Thermophilic and Mesophilic F-ATP Synthase Molecular Motors.

ABSTRACT: The angular velocities of ATPase-dependent power strokes as a function of the rotational position for the A-type molecular motor A₃B₃DF, from the Methanosarcina mazei Gö1 A-ATP synthase, and the thermophilic motor α₃β₃γ, from Geobacillus stearothermophilus (formerly known as Bacillus PS3) F-ATP synthase, are resolved at 5 μs resolution for the first time. Unexpectedly, the angular velocity profile of the A-type was closely similar in the angular positions of accelerations and decelerations to the profiles of the evolutionarily distant F-type motors of thermophilic and mesophilic origins, and they differ only in the magnitude of their velocities. M. mazei A₃B₃DF power strokes occurred in 120° steps at saturating ATP concentra

SUBMITTER: Sielaff H 

PROVIDER: S-EPMC5207238 | biostudies-literature | 2016 Dec

REPOSITORIES: biostudies-literature