Multi-line split DNA synthesis: a novel combinatorial method to make high quality peptide libraries.

ABSTRACT:

Background

We developed a method to make a various high quality random peptide libraries for evolutionary protein engineering based on a combinatorial DNA synthesis.

Results

A split synthesis in codon units was performed with mixtures of bases optimally designed by using a Genetic Algorithm program. It required only standard DNA synthetic reagents and standard DNA synthesizers in three lines. This multi-line split DNA synthesis (MLSDS) is simply realized by adding a mix-and-split process to normal DNA synthesis protocol. Superiority of MLSDS method over other methods was shown. We demonstrated the synthesis of oligonucleotide libraries with 1016 diversity, and the construction of a library with random sequence coding 120 amino acids containing few stop codons.

Conclusions

SUBMITTER: Tabuchi I 

PROVIDER: S-EPMC520752 | biostudies-literature | 2004 Sep

REPOSITORIES: biostudies-literature