High-Throughput Resequencing of Maize Landraces at Genomic Regions Associated with Flowering Time.

ABSTRACT: Despite the reduction in the price of sequencing, it remains expensive to sequence and assemble whole, complex genomes of multiple samples for population studies, particularly for large genomes like those of many crop species. Enrichment of target genome regions coupled with next generation sequencing is a cost-effective strategy to obtain sequence information for loci of interest across many individuals, providing a less expensive approach to evaluating sequence variation at the population scale. Here we evaluate amplicon-based enrichment coupled with semiconductor sequencing on a validation set consisting of three maize inbred lines, two hybrids and 19 landrace accessions. We report the use of a multiplexed panel of 319 PCR assays that target 20 candidate loci associated with photoperiod

SUBMITTER: Jamann TM 

PROVIDER: S-EPMC5207663 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature