Comparison in gene expression of secretory human endometrium using laser microdissection.

ABSTRACT:

Background

The endometrium prepares for implantation under the control of steroid hormones. It has been suggested that there are complicated interactions between the epithelium and stroma in the endometrium during menstrual cycle. In this study, we demonstrate a difference in gene expression between the epithelial and stromal areas of the secretory human endometrium using microdissection and macroarray technique.

Methods

The epithelial and stromal areas were microdissected from the human endometrium during the secretory phase. RNA was extracted and amplified by PCR. Macroarray analysis of nearly 1000 human genes was carried out in this study. Some genes identified by macroarray analysis were verified using real-time PCR.

Results

In this study, changes in expression <2

SUBMITTER: Yanaihara A 

PROVIDER: S-EPMC520833 | biostudies-literature | 2004 Sep

REPOSITORIES: biostudies-literature