Modified serial analysis of gene expression method for construction of gene expression profiles of microbial eukaryotic species.

ABSTRACT: Serial analysis of gene expression (SAGE) is a powerful approach for the identification of differentially expressed genes, providing comprehensive and quantitative gene expression profiles in the form of short tag sequences. Each tag represents a unique transcript, and the relative frequencies of tags in the SAGE library are equal to the relative proportions of the transcripts they represent. One of the major obstacles in the preparation of SAGE libraries from microorganisms is the requirement for large amounts of starting material (i.e., mRNA). Here, we present a novel approach for the construction of SAGE libraries from small quantities of total RNA by using Y linkers to selectively amplify 3' cDNA fragments. To validate this method, we constructed comprehensive gene expression profiles

SUBMITTER: Coyne KJ 

PROVIDER: S-EPMC520878 | biostudies-literature | 2004 Sep

REPOSITORIES: biostudies-literature