Dual-Modality Imaging of Prostate Cancer with a Fluorescent and Radiogallium-Labeled Gastrin-Releasing Peptide Receptor Antagonist.

ABSTRACT: Gastrin-releasing peptide (GRP) receptors (GRPr) are frequently overexpressed in human prostate cancer, and radiolabeled GRPr affinity ligands have shown promise for in vivo imaging of prostate cancer with PET. The goal of this study was to develop a dual-modality imaging probe that can be used for noninvasive PET imaging and optical imaging of prostate cancer.

Methods

We designed and synthesized an IRDye 650 and DOTA-conjugated GRPr antagonist, HZ220 (DOTA-Lys(IRDye 650)-PEG₄-[D-Phe⁶, Sta¹³]-BN(6-14)NH₂), by reacting DOTA-Lys-PEG₄-[D-Phe⁶, Sta¹³]-BN(6-14)NH₂ (HZ219) with IRDye 650 N-hydroxysuccinimide (NHS) ester. Receptor-specific binding of gallium-labeled HZ220 was characterized in PC-3 prost

SUBMITTER: Zhang H 

PROVIDER: S-EPMC5209642 | biostudies-literature | 2017 Jan

REPOSITORIES: biostudies-literature